Info

115.53 ±42.43 19.70 ±2.15 82.03 ± 3.01 46.73 ± 8.23

131.97 ± 29.85 23.73 ±2.61 66.79 ± 9.88 8.07 ± 1.06

Proliferation of murine splenocytes (106 cell/ml) and murine leukaemic LB cells (5.105 cell/ ml) was meausred by (3H)-thymidine uptake. The cells were incubated for 24 h at 37°C, pulsed with 1 pCi (3H)-thymidine for further 24 h. The cells were stimulated with Concanavalin A (ConA; 7 pg/ml) or with LPS (20 ^g/ml). Control cellular growth in absence of L. cuneifolia extract was taken as 100%. Proliferation was calculated as

(cpmexpenment*100)/cpm control' *

is slightly stimulated (Table 2), while mitogen-activated splenocytes were inhibited, an effect more pronounced for the mitogenic lectin Concanavalin A than for lipopolysaccharide. In contrast to normal murine splenocytes, leukaemic LB cells were strongly inhibited by L. cuneifolia extracts (Table 2) (Fernández et al., 1998), indicating that leukemic cells are more sensitive towards the drug than normal cells. These cells were killed by apoptosis (Figure 7).

Since the inducible form of the arginine-dependent enzyme nitric oxide synthase generates toxic amounts of nitric oxide, which enables the activated macrophage to destroy tumour cells and microorganisms, L. cuneifolia effect on nitric oxide production has been evaluated (Cui et al., 1994). Murine macrophages from normal mice collected after thyoglycolate and Concanavalin A stimulation were cultured alone or in the presence of lipopolysaccharide or variable doses of recombinant murine interferon-g. In all cases, L. cuneifolia acellular extracts are capable of enhancing nitric oxide production (Fernández et al., 1998) (Table 3).

Figure 7 Apoptosis in murine splenocytes (106 cell/ml) and murine leukaemic LB cells (5.105 cell/ ml) treated with L. cuneifolia extract for 48 h. Apoptotic nuclei were measured by UV microscopy (epillumination) using acridine orange and ethidium bromide. Results are means ± SD from 5 different experiments and are given as % of cells (total number of cells with apoptotic nuclei per total number of cellsx100 from 5 different experiments). Apoptosis was verified by the characteristic DNA labelling in agarose gel (data not shown).

Figure 7 Apoptosis in murine splenocytes (106 cell/ml) and murine leukaemic LB cells (5.105 cell/ ml) treated with L. cuneifolia extract for 48 h. Apoptotic nuclei were measured by UV microscopy (epillumination) using acridine orange and ethidium bromide. Results are means ± SD from 5 different experiments and are given as % of cells (total number of cells with apoptotic nuclei per total number of cellsx100 from 5 different experiments). Apoptosis was verified by the characteristic DNA labelling in agarose gel (data not shown).

Table 3 Nitric oxide production by murine macrophages.

L. cuneifolia

alone

+ LPS

+ IFNy

+ IFNy

+ IFNy

Was this article helpful?

0 0
Berry Boosters

Berry Boosters

Acai, Maqui And Many Other Popular Berries That Will Change Your Life And Health. Berries have been demonstrated to be some of the healthiest foods on the planet. Each month or so it seems fresh research is being brought out and new berries are being exposed and analyzed for their health giving attributes.

Get My Free Ebook


Post a comment