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Bassoon-GFP

Figure 16.3. Visualization of PTVs in Immature Primary Neurons. (A) Immunofluorescence localization of Bassoon in an axon. The punctate distribution most likely represents Bassoon bound to PTVs. The inset shows a three-fold magnification, the scale bar represents 500 nm. (B) Fluorescence of GFP-Bassoon in a transfected neuron. The recombinant protein is distributed similar to the endogenous protein. The size and distribution of the puncta are reminiscent of the endogenous protein. Such GFP-Bassoon puncta are highly mobile42 and can be recruited to nascent synapses38. The neurons were maintained for 4 days in culture.

Figure 16.3. Visualization of PTVs in Immature Primary Neurons. (A) Immunofluorescence localization of Bassoon in an axon. The punctate distribution most likely represents Bassoon bound to PTVs. The inset shows a three-fold magnification, the scale bar represents 500 nm. (B) Fluorescence of GFP-Bassoon in a transfected neuron. The recombinant protein is distributed similar to the endogenous protein. The size and distribution of the puncta are reminiscent of the endogenous protein. Such GFP-Bassoon puncta are highly mobile42 and can be recruited to nascent synapses38. The neurons were maintained for 4 days in culture.

The appearance of cadherins on PTVs is of particular interest as a recent study has shown that cadherins in conjunction with P-catenin is involved in presynaptic assembly and localization of SVs44. Deletion of P-catenin in hippocampal primary neurons results in a reduced size of reserve pool SVs and in an impaired response to repetitive synaptic stimulation. This cadherin/p-catenin function seems to involve the PDZ binding motif of P-catenin and its interaction with a downstream PDZ domain protein.

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