Results

The effect of NM on in situ 26S proteasome activity

After treatment with NM or iron the accumulation of ZsGFP was observed in cyto-

Fig. 1. Effects of NM on the in situ activity of 26S proteasome in SH-SY5Y cells transfected with the proteasome sensor vector. The cells were cultured in the presence of 0.1 mg/ml of NM for 3 days. Left: morphological observation of the cells treated with NM. Right: Accumulation of ZsGFP (white arrows) was observed by fluorescence microscopy

Fig. 1. Effects of NM on the in situ activity of 26S proteasome in SH-SY5Y cells transfected with the proteasome sensor vector. The cells were cultured in the presence of 0.1 mg/ml of NM for 3 days. Left: morphological observation of the cells treated with NM. Right: Accumulation of ZsGFP (white arrows) was observed by fluorescence microscopy plasm by fluorescence microscopy (Fig. 1). After 3 days' incubation, the fluorescence intensity in the cells treated with 0.1 mg/ml

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