E. coli cultures infected with bacteriophage M13
Prepare an infected culture as described in Chapter 3, Protocol 2. These cultures should be infected with both the hoped-for recombinant bacteriophage and a control culture infected with nonrecombinant bacteriophage. E. coli cultures, uninfected
Prepare a mock-infected culture by picking an area of the E. coli lawn from the plate that does not contain a plaque as a negative control. Use this culture to monitor the recovery of bacteriophage M13 particles.
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