Vectors and Bacterial Strains

E. coli cultures infected with bacteriophage M13

Prepare an infected culture as described in Chapter 3, Protocol 2. These cultures should be infected with both the hoped-for recombinant bacteriophage and a control culture infected with nonrecombinant bacteriophage. E. coli cultures, uninfected

Prepare a mock-infected culture by picking an area of the E. coli lawn from the plate that does not contain a plaque as a negative control. Use this culture to monitor the recovery of bacteriophage M13 particles.

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