To prepare EDTA at 0.5 M (pH 8.0): Add 186.1 g of disodium EDTA*2H2O to 800 ml of H2O. Stir vigorously on a magnetic stirrer. Adjust the pH to 8.0 with NaOH (approx. 20 g of NaOH pellets). Dispense into aliquots and sterilize by autoclaving. The disodium salt of EDTA will not go into solution until the pH of the solution is adjusted to approx. 8.0 by the addition of NaOH.
Add 25 ml of concentrated HCl (11.6 N) to 91 ml of sterile H2O. Store the diluted solution at room temperature (2.5 N solution).
HEPES-buffered saline buffer
10 mM HEPES pH (7.4) 150 mM NaCl 3 mM EDTA 0.005% (v/v) Tween-20
This buffer is commercially available from BIAcore. NaCl
To prepare a 5 M solution: Dissolve 292 g of NaCl in 800 ml of H2O. Adjust the volume to 1 liter with H2O. Dispense into aliquots and sterilize by autoclaving. Store the NaCl solution at room temperature.
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