Preparation of 01 M Sodium Phosphate Buffer at

25 UC

pH

volume OF 1 m Na2HP04 (ml)

VOLUME of 1 M NaHjPO^ (ml)

7.9

92.1

6.0

12.0

88.0

6.2

17.8

82.2

6 A

25.5

74,5

6.6

35.2

64.8

6.8

46.3

517

7.0

57.7

42.3

7.2

6S.4

31.6

7.4

77.4

22.6

7.6

84.j

15,5

7.8

89.6

10.-1

8.0

33.2

6j8

Dilute the combined I V stock sq]u:loti5 to 1 liter with distilled H^O. pH Ls Tabulated according to tint Haiderson Hassclbalch ecua tlon:

f)]|=pK' h ][]fr [ fprtil []il a ccept rir)~| pnotondonor J

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