Neutralizing Solution for Neutral Transfer

For double-stranded DNA targets only.

Rich K LB

NZCYM

For solid medium, please see Media Containing Agar or Agarose. SM

NaCl, 5.8 g MgSO4*7H2O, 2 g 1 M Tris-Cl (pH 7.5), 50 ml H2O, to 1 liter Gelatin

If needed, add 5 ml gelatin solution (2%). Sterilize the buffer by autoclaving for 20 minutes at 15 psi (1.05 kg/cm2) on liquid cycle. After the solution has cooled, dispense 50-ml aliquots into sterile containers. SM may be stored indefinitely at room temperature. Discard each aliquot after use to minimize the chance of contamination.

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