a. Wearing gloves, remove the bag from the water bath, remove the outer bag, and immediately cut off one corner of the inner bag. Pour out the hybridization solution into a container suitable for disposal of radioactivity, and then cut the bag along the length of three sides.
b. Remove the membrane and immediately submerge it in a tray containing several hundred milliliters of 2x SSC and 0.5% SDS (i.e., approx. 1 ml/cm2 membrane) at room temperature. Agitate the tray gently on a slowly rotating platform.
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