For hybridization in a heatsealable bag

a. Wearing gloves, remove the bag from the water bath, remove the outer bag, and immediately cut off one corner of the inner bag. Pour out the hybridization solution into a container suitable for disposal of radioactivity, and then cut the bag along the length of three sides.

b. Remove the membrane and immediately submerge it in a tray containing several hundred milliliters of 2x SSC and 0.5% SDS (i.e., approx. 1 ml/cm2 membrane) at room temperature. Agitate the tray gently on a slowly rotating platform.

Was this article helpful?

0 0

Post a comment