To prepare a 10 M solution in 1 liter, dissolve 770 g of ammonium acetate in 800 ml of H2O. Adjust volume to 1 liter with H2O. Sterilize by filtration. Alternatively, to prepare a 100-ml solution, dissolve 77 g of ammonium acetate in 70 ml of H2O at room temperature. Adjust the volume to 100 ml with H2O. Sterilize the solution by passing it through a 0.22-jm filter. Store the solution in tightly sealed bottles at 4°C or at room temperature. Ammonium acetate decomposes in hot H2O and solutions containing it should not be autoclaved.
Was this article helpful?