Alkaline Gelloading Buffer

300 mM NaOH 6 mM EDTA

18% (w/v) Ficoll (Type 400, Pharmacia) 0.15% (w/v) bromocresol green 0.25% (w/v) xylene cyanol

For a 6x buffer. EDTA

To prepare EDTA at 0.5 M (pH 8.0): Add 186.1 g of disodium EDTA*2H2O to 800 ml of H2O. Stir vigorously on a magnetic stirrer. Adjust the pH to 8.0 with NaOH (approx. 20 g of NaOH pellets). Dispense into aliquots and sterilize by autoclaving. The disodium salt of EDTA will not go into solution until the pH of the solution is adjusted to approx. 8.0 by the addition of NaOH.

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