dry air dry air
roller drum coverslip and fibrin clot
culture medium semipermeable membrane
FIGURE 1 Preparation of organotypic brain slice cultures. The tissue of interest is first dissected free from the neonatal brain, as illustrated for the hippocampus, and thin sections are cut under aseptic conditions using either a tissue chopper or a vibratome. A: In the roller-tube method, the slices are attached to cleaned glass coverslips in a film of clotted fibrin or chicken plasma; the slices are then placed in sealed test tubes and placed on a roller drum in a dry-air incubator. B: In the membrane method, the slices are placed on semipermeable membranes in multiwell plates so that they are at the gas-air interface and then maintained in a CO2 incubator.
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