B

FIGURE 7—6 Orthograde transport of biocytin reveals callosal projection in coronal slices. A: A biocytin crystal (-0.5 mm diameter) was placed in layer V of the contralateral cingulate cortex (right side, crystal placement not shown in this figure). The slice was incubated for 3 hours in an interface chamber and then processed to obtain a final horseradish peroxidase reaction product. The arrows in A indicate labeling of the callosal fibers. The square area marked in A is expanded in B. B: A dark-field image showing the callosal fibers coursing upward through the ipsilateral layer V.

FIGURE 13—1 Schematic representation of a system for administration of ethanol by inhalation modified after Ruwe et al. (1986). Ethanol (95%) is delivered by a solvent metering pump into a 250-ml vaporization chambers. The airtight vaporization chamber is maintained at 37° C by a water bath. Air is delivered into the vaporization chamber with an air pump to provide a flow rate of 2.5 to 4 liters per minute. Animals are exposed to ethanol-vapor concentrations of 7 to 35 mg/liter of air in a Plexiglas experimental chamber. A food tray and water bottle are securely affixed to the experimental chamber, giving the animals free access to food and water. The sample port allows air within the experimental chamber to be sampled for determination of ethanol concentrations.

FIGURE 13—1 Schematic representation of a system for administration of ethanol by inhalation modified after Ruwe et al. (1986). Ethanol (95%) is delivered by a solvent metering pump into a 250-ml vaporization chambers. The airtight vaporization chamber is maintained at 37° C by a water bath. Air is delivered into the vaporization chamber with an air pump to provide a flow rate of 2.5 to 4 liters per minute. Animals are exposed to ethanol-vapor concentrations of 7 to 35 mg/liter of air in a Plexiglas experimental chamber. A food tray and water bottle are securely affixed to the experimental chamber, giving the animals free access to food and water. The sample port allows air within the experimental chamber to be sampled for determination of ethanol concentrations.

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