Tunel

1. 4% Paraformaldehyde, (w/v), in PBS: see Subheading 2.1., item 1 for PBS recipe.

3. Terminal transferase (Boehringer Mannheim, Mannheim, Germany), which comes with the terminal transferase buffer as a 5X stock and 25 mM CoCl2.

4. Deoxy uridine triphosphate (dUTP) and biotin-16-deoxy uridine triphosphate (Biotin-16-dUTP) (Boehringer Mannheim).

5. Streptavidin-fluorescein reagent (Amersham, Little Chalfont, UK).

6. Streptavidin-horseradish peroxidase conjugate reagent (Amersham).

7. 1,4-Diazabicyclo [2,2,2] octane (DABCO) (Sigma).

Was this article helpful?

0 0

Post a comment