The Frog as a Mouse

6. The transgenesis procedure described here compares very favorably with those developed for mouse or zebrafish. Embryos expressing plasmids nonmosaically can be obtained in high numbers directly, and since the embryos are not chi-meric, breeding of animals is not required. In addition, the cost of studies involving transgenic frog embryos will be considerably lower than that required for similar studies in mammals. In fact, the frog may prove useful for the study of regulation of mouse promoters. Limited studies have shown that mouse promoters are regulated appropriately in other systems and vice versa (23-28). Therefore, initial promoter mapping and analysis may be done more easily and effectively in the frog than in the mouse.

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