Synthesis of Digoxygenin DIG or Fluorescein12 Utp Fitc Labeled Riboprobes

1. RNA polymerases, manufacturer's buffers, and 200 mMDTT (Promega, Southampton, UK, and Boehringer Mannheim). Aliquots of 200 mM DTT are stored at -20°C.

2. 10X DIG and/or fluorescein RNA labeling nucleotide mixes (Boehringer Mannheim).

3. RNasin (Boehringer Mannheim).

4. RNase-free DNase-1 (Boehringer Mannheim).

5. TE, pH 7.0: 10 mM Tris-HCl, pH 7.0, 1 mM ethylenediamine tetraacetic acid, disodium salt (EDTA), pH 8.0, diluted from stocks dissolved in DEPC-treated water.

6. 4 M Lithium chloride (prepared with DEPC-treated water).

7. 70% (v/v) Ethanol (prepared with DEPC-treated water).

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