Solutions

1. Chick Ringer solution: 7.2 g/L NaCl, 0.23 g/L CaCl, 0.37 g/L KCl, pH 7.2. Immediately before use, add 1 in 50 of penicillin/streptomycin solution (Pen/ Strep; Gibco, Grand Island, NY).

2. 1X Hanks' Balanced Salt Solution (HBSS) (Gibco).

3. Dispase (Grade 1; Boehringer Mannheim, Mannheim, Germany). Dilute at 1 mg/mL in HBSS containing 50 pg/mL Deoxyribonuclease (Sigma, St. Louis, MO).

4. Ink solution for subblastodermal injection to visualize embryos. Ink is Pelikan drawing ink A, shade 17 (black). Dilute at 1 in 15 to 1 in 20 in Ringer solution containing Pen/Strep.

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