Scoring the Results

The result of a mesoderm induction assay may be score in several ways. In increasing order of complexity, these are:

1. Observation of gastrulation-like movements. Normally, isolated animal pole tissue "rounds up" after being dissected from the embryo, and it forms a sphere. Treatment with a mesoderm-inducing factor causes the animal pole cells to undergo gastrulation-like movements. However, and the animal caps elongate in a characteristic fashion (18; Fig. 2). Activin and other members of the TGF-P family cause more dramatic gastrulation movements than do members of the FGF family (6,18,19). Elongation movements are visible after approx 4 h of culture.

Was this article helpful?

0 0

Post a comment