RNA Dot Blot

1. Formamide (Gibco-BRL).

2. Formaldehyde, 37% solution (BDH, Leicester, UK).

3.1 M Sodium phosphate buffer: Dissolve 70.5 g of anhydrous Na2HPO4 in dH2O, add 4 mL of phosphoric acid and make up to 1 L with H2O. Filter sterilize.

4. Hybond N membrane (Amersham, Buckinghamshire, UK).

5. Prehybridization/hybridization buffer (40 mL, made fresh): weigh out 0.4 g of bovine serum albumin (Sigma), and dissolve in 14 mL of 1 M phosphate buffer. Add 12 mL of formamide and 14 mL 20% SDS.

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