Removal of loxPFlanked Genomic Segment In Situ by Transiently Expressing the Cre Recombinase in F1 Preimplantation Stage

An alternative method for excising loxP-flanked DNA is to express the Cre recombinase in an early embryo transiently (42).

1. Briefly, germline transmission of the primary loxP-flanked DNA sequence is achieved.

2. Then a Cre-expressing plasmid is injected in circular form into the pronucleus of zygotes produced by a cross between males carrying the loxP-flanked sequence and wild-type females.

3. Injected embryos are transferred into pseudopregnant recipients.

4. Offspring are screened for the required excision event.

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