An alternative method for excising loxP-flanked DNA is to express the Cre recombinase in an early embryo transiently (42).
1. Briefly, germline transmission of the primary loxP-flanked DNA sequence is achieved.
2. Then a Cre-expressing plasmid is injected in circular form into the pronucleus of zygotes produced by a cross between males carrying the loxP-flanked sequence and wild-type females.
3. Injected embryos are transferred into pseudopregnant recipients.
4. Offspring are screened for the required excision event.
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