Reagents for Poly A Selection

1. 2X Loading buffer: 40 mM Tris-HCl, pH 7.6, 1 M NaCl, 2 mM EDTA, 0.2% SDS.

3. Loading buffer (low-salt): 40 mM Tris-HCl, pH 7.6, 0.1 M NaCl, 2 mM EDTA, 0.2% SDS.

4. Elution buffer: 10 mM Tris-HCI, pH 7.5, 1 mM EDTA, 0.05% SDS.

6. 100% Ethanol (reserved for RNA use).

7. 70% Ethanol (reserved for RNA use).

8. Oligo (dT) cellulose, Type 2 (Collaborative Research, Los Altos, CA, cat. # 20002).

9. Quik-Sep columns (Isolab, Akron, OH, cat. # QS-Q).

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