Preparation of Primary Cultures of CEFs

Primary cultures of CEFs, compatible with the retroviral subtype being used, are required for the production of high-titer viral stocks and/or infected cells for grafting. All retroviral subtypes, except E, can be grown on CEFs made from line 0 embryos, which contain no endogenous retroviruses. Line 15bj embryos are susceptible to infection by E subgroup viruses (see Note 4).

All instruments should be sterilized by autoclaving (where possible), or by rinsing in alcohol and sterile technique used throughout. Wear gloves (ethanol-sterilized).

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