Preparation of Host and Donor Embryos and Grafts

1. 70% Alcohol for sterilization.

2. Disposable syringes (1- or 2-mL) and needles (0.6-0.8 mm in diameter).

3. Transparent Scotch tape (5 cm in width) (see Note 5).

4. Paraffin 60°C and a thin paint-brush.

5. Physiological liquids: PBS or Tyrode solution.

6. Antibiotics: penicillin and streptomycin (see Note 7).

7. Proteolytic enzymes (pancreatin) (see Note 7).

9. Pasteur pipets.

10. Glass micropipets hand-drawn from Pasteur pipets and equipped with plastic tubes for mouth use (Tygon) (see Note 8).

11. Small glass dishes (salières) normal or containing a plastic base for dissections (rhodorsyl, Rhône-Poulenc, France) (see Note 9) and insect pins.

12. Indian ink (Pelikan for drawing) (see Note 10).

13. Microscalpels and their holders (see Note 11).

14. Small curved scissors, iridectomy (Pascheff-Wolff) scissors, and thin forceps (Swiss Dumont no. 5 forceps) (Moria Instruments, Paris).

15. Optical equipment: stereomicroscope equipped with a zoom from x6 to x50.

16. Optic fibers for object illumination (see Note 12).

Was this article helpful?

0 0

Post a comment