Preparation of Embryos for Confocal Microscopy

1. For confocal microscopy embryos are analyzed in whole mount. Embryos that are 8.5 d old and older need to have the extraembryonic tissues dissected away. Embryos that are 6.5 and 7.5 d old are analyzed intact.

2. Embryos are mounted flat under a coverslip (coverglass number 11/2). To allow sufficient space for the embryos between the slide and the coverslip, small blue-tac feet are placed at the four corners of the coverslip (see Note 21).

3. The slide is filled with PBS and sealed with nail polish.

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