Postantibody Washes and Histochemistry

1. NTMT, pH 9.5: 100 mM NaCl, 100 mM Tris-HCl, pH 9.5, 50 mM MgCl2, 0.1% Tween-20, 2 mM levamisole (prepared freshly on day of use from concentrated stock solutions of the individual components).

2. Nitroblue tetrazolium (NBT) and 5-bromo-4-chloro-3-indolyl phosphate (BCIP/ x-phosphate) both from Boehringer.

3. PBT containing 20 mM EDTA.

4. NTMT, pH 8.0: 100 mM NaCl, 100 mM Tris-HCl, pH 9.5, 50 mM MgCl2, 0.1% Tween 20, 2 mM levamisole (prepared freshly on day of use from concentrated stock solutions of the individual components).

5. Fast red TR/naphthol AS-MX (Sigma).

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