Obtaining Xenopus Embryos by Artificial Fertilization

1. Xenopus laevis adult females and males: These may be obtained from a variety of sources; up-to-date details of suppliers throughout the world are available on the Xenopus molecular marker resource: URL http://vize222.zo.utexas.edu/. Instructions for keeping Xenopus are beyond the scope of this chapter; interested readers may refer to ref. 13.

2. Pregnant mare serum gonadotrophin (PMSG; Sigma, St. Louis, MO).

3. Human chorionic gonadotrophin (HCG; Sigma).

4. 2-mL Syringe and 23- to 25-gage needles.

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