1. Following surgery, the mice or rats are caged individually and left to recuperate for at least 3 wk prior to the presentation of the first female.

2. Check that the males are vasectomized, but capable of mating by presenting each male with a different female for 2 wk running. Successful mating is confirmed by the presence of a copulatory plug in the female and male sterility by the females not becoming pregnant. Any male not meeting both criteria should be replaced.

3. Vasectomized males should be presented with females only once every 2 d at the most.

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