1. Following the production of transgenic founders, mate the animals as soon as possible to establish a transgenic line. Analysis of the founder phenotype should not begin until the transgene has passed successfully to a subsequent generation. In vitro fertilization may be necessary if the founders do not mate.
2. Unless the animals are kept in an SPF unit, sporadic microbial infections will occur. A six-monthly check of the colony's health status by a veterinarian is advisable.
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