Neuronal Trancing Using Lipophilic Membrane Dyes Fluorescent Dextrans and Horseradish Peroxidase HRP

1. Paraformaldehyde.

2. Phosphate-buffered saline (PBS).

5. Glycerol.

7. Physiological saline (137 mM NaCl, 5 mM KCl, 2 mM CaCl2, 1 mM MgCl2, 1 mM NaH2pO4, 5 mM HEPES, 11 mM glucose, pH 7.4).

8. Fluorescent dextran, Molecular Probes product numbers D-3308, D-3306.

9. HRP, Boehringer Grade 1 lyophilized (Boehringer Mannheim, Indianapolis, IN).

11. Diaminobenzidine (DAB).

12. Hydrogen peroxide.

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