Materials

1. Avertin: A 100% stock is made by mixing 10 g of tribromoethylalcohol with 10 mL of tertiary amyl alcohol. The 2.5% working solution is diluted from stock in sterile water. Both are stored at 4°C, protected from light.

2. Sterile 1-mL disposable syringe.

3. Sterile 0.5 x 16 mm disposable needles.

4. Animal balance

1. CRC: Mix one part Dormicum™ (5 mg/mL midazolam, Hoffmann-La Roche, Basel, Switzerland), one part Hypnorm™ (l0 mg/mL fluanisone and 0.2 mg fen-

tanyl, Janssen Pharmaceuticals, Beerse, Belgium), and two parts sterile water. The madeup solution is stable at room temperature for 1 wk.

2. Sterile 1-mL disposable syringe.

3. Sterile 0.5 x 16 mm disposable needles.

4. Animal balance.

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