Materials

Digoxygenin-11-UTP (Boehringer-Mannheim 1209 256) Ribonucleotides 1 M DTT RNasin

RNA Polymerase (SP6,T3, T7) DNase I (RNase-free) MOPS EGTA MgSO4 Formaldehyde Methanol

Torula RNA

Heparin

Tween-20

CHAPS

EDTA

Maleic acid (optional) NaCl

Lamb serum MgCl

Tris, pH 9.5 Levamisol

From: Methods in Molecular Biology, Vol. 97: Molecular Embryology: Methods and Protocols Edited by: P. T. Sharpe and I. Mason © Humana Press Inc., Totowa, NJ

Formamide 20X SSC

Triton X-100

Nitro blue tetrazolium (NBT) 5-bromo-4-chloro-3-indolyl-phosphate (BCIP) Dimethyl formamide

Other reagents and optional equipment are listed in the text where appropriate. Working concentrations and storage conditions for stock solutions are also detailed.

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