Materials

1. Follicle-stimulating hormone (FSH): Either Folligon (Intervet Laboratories Ltd, Cambridge UK) or Pregnant Mares Serum Gonadotrophins (PMSG; Sigma, St. Louis, MO; cat. no. GH879) can be used. Working solution: 50 IU/mL made up with sterile water or 0.9% (w/v) NaCl. Store frozen at 4°C in 1-mL aliquots.

2. Human chorionic gonadotrophin (hCG): Either Chorulon (Intervet Laboratories Ltd., Cambridge, UK) or hCG (Sigma CG-5) can be used. Working solution: 50 IU/mL made up with sterile water or 0.9% (w/v) NaCl. Store frozen at 4°C in 1-mL aliquots.

3. Sterile 1-mL disposable syringes and 25-gage needles.

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