All glassware and instruments should be clean to tissue-culture standards and autoclaved or heat-sterilized the day before use. AnalaR® reagents are recommended for the solutions, which should be made up and autoclaved the day before use. If the technique is used as a classroom exercise, it is quite sufficient to use Howard's Ringer. However, if it is employed as a tool to do serious research, the use of a phosphate-buffered salt solution, such as the one described later, is advised.
Explantation of the cultures may be performed in a quiet draft-free place on the laboratory bench if a tissue culture hood is not available.
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