Irrespective of how mutant embryos are produced, a similar protocol can be employed for the use of markers to detect mutant phenotypes. Zebrafish offer some unique alternatives in the detection of specific phenotypes. The ease with which embryos can be manipulated, the large brood size, optically clear embryos and reliability of procedures to detect mRNA or protein in fixed embryos allow the use of "markers" of specific developmental processes. Several markers may be used in combination. A typical protocol for marker-based screening is outlined below. Obviously, the stage of embryos fixed will vary between those markers used (see Note 8).
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