Some transgenic animals may be infertile owing to an inability to mate or to rear a litter. With mice, in vitro fertilization (IVF) can be used to continue the line. the technique involves superovulating females and fertilizing the eggs with sperm taken from the epididymis of males. IVF eggs are then transferred to a 0.5-d pc pseudopregnant recipient female. The strain of mouse used should be the same as those used for the original transgenic mouse production (e.g., C57BL/6J X CBA/JF1). Males should be more than 6 wk of age and preferably studs rested the previous night. Females should be at least 4 wk of age. The described protocol employs a single male and 10 females.
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A Beginner's Guide to Healthy Pregnancy. If you suspect, or know, that you are pregnant, we ho pe you have already visited your doctor. Presuming that you have confirmed your suspicions and that this is your first child, or that you wish to take better care of yourself d uring pregnancy than you did during your other pregnancies; you have come to the right place.