Immunohistochemical or In Situ Analysis of Cultures

1. 0.2 M Phosphate buffer, pH 7.4 (PB): 6 g/L NaH2PO4, 21.8 g/L Na2HPO4.

2. 4% Paraformaldehyde in 0.12 M PB, pH 7.4: heat 20 mL H2O to 80°C. Add 2 g paraformaldehyde, invert, and add two to three drops 1 M NaOH. Invert or stir until solution clears. Make up to 50 mL with 0.2 M PB and filter.

4. OCT (TissueTek) and gelatin-subbed slides or Superfrost Plus slides (Fisher).

5. Glycergel (DAKO).

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