Hybridization to Tissue Sections

Proteinase K (Boehringer Mannheim) 20 mg/mL in water stored in single-use (20 ||L) aliquots at -20°C.

TE pH 8.0 (10 mM Tris-HCl, pH 8.0, 1 mM EDTA, pH 8.0) diluted in DEPC

water and prepared from concentrated stocks dissolved in DEPC water.

Triethanolamine (Merck BDH).

Acetic anhydride (Merck BDH).

Hybridization buffer: 50% (v/v) deionized formamide, 0.3 M NaCl, 20 mM Tris-HCl, pH 8.0, 5 mM EDTA, pH 8.0, 10% (w/v) dextran sulfate, 1X Denhardt's solution, 0.5 |g/mL yeast tRNA. Store in aliquots at -70°C.

Was this article helpful?

0 0

Post a comment