Green Fluorescent Protein Expression in Live Embryos 2425

The cDNA encoding GFP from Aequorea victoria, provides an alternative, more convenient marker for living embryos than the P-gal substrates described above (20). GFP produces fluorescence without the need to add an exogenous substrate. GFP absorbs blue light (maximally at 395 nm with a minor peak at 470 nm) and emits green light (peak emission at 509 nm with a shoulder at 540 nm).

The fluorescence is very stable with virtually no photobleaching. Embryos can be viewed directly with a fluorescein filter set.

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