In our experience, R1 ES cells (17) can be propagated on both types of plates without losing their totipotency or their ability to contribute to the germline (see Note 7).
1. To prepare gelatinized plates rinse the surface of tissue-culture dishes with a 0.1% gelatin solution (approx 100 |L/well of 96-well plates, or 3 mL for 6-cm plates, 5 mL for 10-cm plates).
2. Aspirate off the excess gelatin, and then allow the surface of the plates to dry a little (2-4 min).
3. Add fresh medium to the plates, and place them into the incubator until required.
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