FDG see Note 21 Visualization of Gal Activity in Live Embryos

1. Embryos are dechorionated and stained in a solution containing 2 mM FDG and 20% DMSO in ddH2O for exactly 2 min and 30 s at 33°C. Note that older embryos that have muscular movements may be anesthetized in tricaine to aid viewing and photography.

2. Wash extensively with fish water.

3. Examine with a low-power objective (5X) on a fluorescence microscope with an FITC filter set.

4. False positives can develop after 30-40 min. Some tacZ-expressing embryos may be overlooked if expression is not very strong.

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