Enzymes for cDNA Library Construction

1. AMV reverse transcriptase (Seikagaku America #120248-1 [St. Petersburg, FL]).

2. DNA polymerase I, endonuclease-free (BMB # 642-711).

3. RNase H (Pharmacia #27-0894-02 [Milwaukee, WI]).

4. T4 DNA polymerase (Pharmacia #27-0718-02).

5. T4 polynucleotide kinase (Pharmacia #27-0734-01).

6. T4 DNA ligase (Pharmacia #27-0870-01).

7. T4 RNA ligase (Pharmacia #27-0883-01).

8. Escherichia coli DNA ligase (BMB #862 509).

9. EcoRI methylase (New England Biolabs, NEB #211S [Tozier, MA], S-adenosyl methionine is supplied with the enzyme.

10. Human placental ribonuclease inhibitor (Pharmacia RNA guard #27-0815-01).

11. EcoRI high concentration (BMB #200-310).

12. XhoI—high concentration (BMB #703-788).

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