Developing and Mounting Slides

D19 developer (Kodak, Paris, France): Dissolve 64 g D19 developer in 400 mL water.

This can be used several times on the day of preparation, but discard after use.

Stop solution: 350 mL of 1% (v/v) acetic acid/1% (v/v) glycerol.

Fixing solution: 350 mL of 30% (w/v) sodium thiosulfate.

0.25% Toluidine blue (Sigma) filtered through a Whatman general-purpose filter.

Depex mounting medium (Merck BDH).

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