Detection of Mutant Phenotypes

1. Microscopy: All morphological and marker screening utilizes simple light microscopy via a dissecting scope. We use Zeiss Stemi SR microscopes. However, any microscope with the capability of translucent illumination and up to 5X objectives would be adequate. Translucent lighting is essential for clear optical inspection of structures of the embryo.

2. In situ hybridizations: In situ probes and protocols use standard methods (see Chapter 31 and refs. 12,13).

3. Antibodies: Antibodies are either bought commercially or are available from different laboratories on request. We utilize standard methods for protein detection (12).

4. Alcian blue: A stock solution of alcian blue stain is made by dissolving powder to 0.1% (w/v) in 70% (v/v) Et0H/30% (v/v) glacial acetic acid.

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