Detection of lacZ Positive Cells at the Ultrastructural Level

1. Fixative: 4% paraformaldehyde, 0.5% electron microscopy grade glutaraldehyde in 0.1 M phosphate buffer, pH 7.2-7.4.

2. Post fixation solution: 4% paraformaldehyde, 2% glutaraldehyde in 0.1 M phosphate buffer, pH 7.4.

3. Staining and dehydration of tissue samples:

a. 1% Osmium tetroxide in 0.1 M phosphate buffer, pH 7.4.

b. 6- and 24-Well plastic tissue culture plates.

c. 0.1 M Sodium acetate.

d. 1% Uranyl acetate.

e. 0.1 N Sodium acetate.

4. Embedding compounds:

a. Araldite.

c. High-quality acetate paper (see Note 13).

d. Hole-punch.

e. Small weights.

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