Culture of Fertilized One Celled Eggs 61 Introduction

For the purpose of making transgenic rats and mice, fertilized one-celled embryos have to be maintained outside of the natural environment for up to 36 h. Fertilized eggs are collected at approx 12 h pc (coitus will normally occur at about 0030 h) and are injected at some point during the following 12 h. The eggs are then returned to the natural environment of the pseudopregnant surrogate mother. This procedure occurs either immediately after microinjection while the eggs are still in the one-celled stage or after overnight culture when the eggs are transferred in the two- celled stage.

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