Combining Homologous and Site Specific Recombination

Homologous recombination in ES cells allows the precise disruption (knockout) of a target gene. Many new approaches require a defined alteration of a gene or the genome. By combining the homologous and site-specific recombinations, we are now in the position of creating most desired alterations in the mouse genome (12,13). In the following section, we will briefly introduce some of the most important current applications. The list will not be complete, since novel applications for the use of this system are continuously being reported.

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