Zebrafish have been used in studies on the effects of CNS development (35), heart development (36), eye development (37), and fin regeneration (38). In all these cases, tRA is administered to the animals in the tank water. A tRA in DMSO aliquot is used at an appropriate concentration to give a final concentration of 10-6 M or 10-7 M when diluted in, say, 5 mL of zebrafish water. Many embryos can be treated at once in a 5-mL Petri dish. For studies of CNS and heart development, embryos are treated for 1 h at 50% epiboly; for eye development for 4 h at 10.5 h of development, and for fin regeneration, adults are treated for 4 or 7 d.
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