An alternative to beads is to use the RA soaked onto paper (29).
1. A piece of absorbent paper (blotting paper, newsprint, and so forth) is placed into a solution of RA in DMSO (see Subheading 2.1.) for 10 min and then washed in medium to remove excess RA.
2 Cut the paper into small pieces (500-^m squares).
3. Insert a square into a slit made in the anterior margin of the limb bud with a tungsten needle. The slit is made perpendicular to the base of the limb opposite intersomite 16/17.
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