Administration in Silastic

1. Cut a block of the appropriate size.

2. Window eggs at the appropriate stage.

3. Tear the vitelline membrane adjacent to the part of the embryo to be treated with a tungsten needle.

4. Place the block adjacent to the embryo.

5. Seal the egg and replace in the incubator.

This method is useful for early embryos, e.g., treating a stage 9/10 hindbrain where the embryo is relatively immobile, but not good for late embryos, which will turn and begin to move. In this case, the block is displaced and the experiment fails.

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