Site Specific Recombination

Recombination can occur between two molecules of DNA that have little sequence similarity. This is known as non-homologous or site-specific recombination. Instead of aligning regions of DNA by sequence homology, the DNA contains a short chi sites Specific sequences on the DNA of eukaryotes where crossovers form

RecA protein Protein involved in recombination and repair of DNA in E. coli that binds single-stranded DNA

site-specific recombination Recombination between two lengths of DNA that are largely unrelated. It involves specific proteins that recognize particular sequences and form crossovers between them. Same as non-homologous recombination

FIGURE 14.06 RecBCD Recognizes Chi Sites

The complex of RecB, RecC, and RecD proteins recognizes the ends of a double-stranded break, and travels along the DNA until reaching the closest chi site. RecD cleaves the backbone of one strand and dissociates from the complex. RecBC continue to unwind the DNA beyond the chi site creating a stretch of single-stranded DNA.

Double stranded break

V RecBCD J

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