Recombination in eukaryotes occurs mostly during the early stages of meiosis. For crossing over to occur between the pairs of homologous chromosomes, double-stranded breaks must be introduced into them—a hazardous procedure. Double-
excisionase Enzyme that reverses DNA integration by removing a segment of dsDNA and resealing the gap. In particular, lambda excisionase removes integrated lambda DNA
Recombination in Higher Organisms 377
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